Journal: Nature Communications

Article Title: Mammalian brain glycoproteins exhibit diminished glycan complexity compared to other tissues

doi: 10.1038/s41467-021-27781-9

Figure Lengend Snippet: Protein lysate from a representative male mouse cortex and cerebellum with human plasma as a positive control was treated with or without PNGase F and visualized using biotinylated lectins (ConA, GNL, PHA-E, AAL, RCA, and SNA) in addition to immunoblotting for actin and staining for total protein. Non-specific binding of lectins to PNGase F is noted by an asterisk (*) near 35 kDa, as shown in the Total Protein stain. Protein blotting of brain lysate with each lectin has been repeated at least three times each with similar results. A schematic with common lectin-binding sites is shown for reference. Source data are provided as a Source Data file.

Article Snippet: Human Brain Cerebral Cortex Whole Tissue Lysate was purchased from Novus Biologicals (#NB820-59182), with 1mg used for glycomic analysis as described below.

Techniques: Clinical Proteomics, Positive Control, Western Blot, Staining, Binding Assay

Journal: Cell reports

Article Title: Intrinsic antiviral immunity of barrier cells revealed by an iPSC-derived blood-brain barrier cellular model

doi: 10.1016/j.celrep.2022.110885

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Brain Cerebral Cortex Tissue Slides (Adult Normal) , Novus Biologicals , Cat#NBP2-77755.

Techniques: Virus, Recombinant, Modification, Membrane, Knock-Out, Clinical Proteomics, Fluorescence, Plasmid Preparation, Western Blot, Mutagenesis, PCR Cloning, Cloning, Software, Cell Culture, Electroporation